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Global in situ profiling of RNA-RNA spatial interactions with RIC-seq, Nat Protoc, 21 May 2021

发布时间:2021年05月21日

Nature Protocols, 21 May, 2021, DOI:

Global in situ profiling of RNA-RNA spatial interactions with RIC-seq

Changchang Cao, Zhaokui Cai, Rong Ye, Ruibao Su, Naijing Hu, Hailian Zhao & Yuanchao Xue

Abstract

Emerging evidence has demonstrated that RNA-RNA interactions are vital in controlling diverse biological processes, including transcription, RNA splicing and protein translation. RNA in situ conformation sequencing (RIC-seq) is a technique for capturing protein-mediated RNA-RNA proximal interactions globally in living cells at single-base resolution. Cells are first treated with formaldehyde to fix all the protein-mediated RNA-RNA interactions in situ. After cell permeabilization and micrococcal nuclease digestion, the proximally interacting RNAs are 3′ end-labeled with pCp-biotin and subsequently ligated using T4 RNA ligase. The chimeric RNAs are then enriched and converted into libraries for paired-end sequencing. After deep sequencing, computational analysis yields interaction strength scores for every base on proximally interacting RNAs in the starting populations. The whole experimental procedure is designed to be completed within 6 d, followed by an additional 8 d for computational analysis. RIC-seq technology can unbiasedly detect intra- and intermolecular RNA-RNA interactions, thereby rendering it useful for reconstructing RNA higher-order structures and identifying direct noncoding RNA targets.

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